Antibodies

Characterization of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) is a critical step in stem cell research. The routine process of validating starting cell populations should be simple, quick, and above all reliable to ensure accurate and consistent results. ESI BIO brings you key antibody formats and live staining tools that are developed specifically for and tested directly on stem cells.

Fast yet reliable verification of pluripotency
ESI BIO’s BioLite™ antibodies allow for direct staining of live cells in culture and therefore eliminate the need to sacrifice valuable material that can be used for downstream experiments.

• Confirm pluripotency marker expression
• Allows for continued cell culture post-staining
• Low endotoxin, sodium azide-free formulation
• No fixation or permeabilization, only one 30 minute incubation required

Figure 1. [A] IF analysis of mouse ES cells. Phase contrast view of cells in culture and the same field of view after staining with BioLite SSEA-1 (Dy- Light 488) Antibody. [B] IF analysis of primary iPS cell colonies at Day 11 of reprogramming adult human fibroblasts. Phase contrast view of cells in culture and the same field of view after staining with BioLite TRA-1-81 (DyLight 488) Antibody. Images were taken at 10X magnification.