Description
Details
DAPT inhibits γ-secretase complex and indirectly inhibits Notch, a γ-secretase substrate. It is known to reduce Aβ40 and Aβ42 levels in human primary neuronal cells (IC50 = 115 and 200 nM for Aβ and Aβ42 respectively) and brain extracts. Its activity causes neural cells to commit to neuronal differentiation and has been shown to reduce colony-forming efficiency in mouse neural stem cells. DAPT has also been shown to promote differentiation of pancreatic cells from human pluripotent stem cells.
Details
Details
| Molecular Weight | 432.46 |
|---|---|
| Molecular Formula | C23H26F2N2O4 |
| Chemical Name | N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine-1,1-dimethylethyl ester |
| CAS Number | 208255-80-5 |
| PubChem Identifier | 5311272 |
| Appearance | White solid |
| Purity | >99% by HPLC analysis |
| Alternative Names | GSI-IX, γ-Secretase Inhibitor IX |
| Solubility | Soluble in DMSO at 100 mM |
| Reconstitution | For a 10 mM concentrated stock solution, reconstitute the compound by adding 1.2 mL of DMSO to the entire contents of vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes. |
| Storage and Stability | Solid: Shipped at room temperature. Store at -20°C. Stable for 6 months when stored as directed. Solution: Following reconstitution, store aliquots in tightly sealed vials at -20°C. Avoid repeated freeze-thaw cycles. |
| References | Androutsellis-Theotokis, A., et al. (2006) Notch signaling regulates stem cell numbers in vitro and in vivo. Nature 442: 823-826. PMID: 16799564. Crawford, T.Q., et al. (2007) The notch response inhibitor DAPT enhances neuronal differentiation in embryonic stem cell-derived embryoid bodies independently of sonic hedgehog signaling. Dev Dyn 236: 886-892. PMID: 17295317. Dovey, H.F., et al. (2001) Functional gamma-secretase inhibitors reduce beta-amyloid peptide levels in brain. J Neurochem 76: 173-181. PMID: 11145990. D’Amour, K.A., et al. (2006) Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat Biotechnol 24: 1392-1401. PMID: 17053790. |
| Technical Documents |