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HyStem®-HP Hydrogels

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The HyStem family of products for research and development is now being exclusively manufactured and sold by Advanced BioMatrix.

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HyStem®-HP Hydrogels - The growth factor delivery matrix. Allows simultaneous growth factor, drug, and cell delivery in vivo less GF required in cell culture for cell signaling. Growth factors added to gel mixture ionically bind with heparin for a slow, more-controllable release. Components include Thiol-modified hyaluronan and heparin (Heprasil®), Thiol-reactive PEGDA crosslinker (Extralink®), Thiol-modified collagen (Gelin-S®), and Degassed, deionized water (DG Water).
Product Name SKU Price
HyStem®-HP Hydrogel Trial Kit, 2.5 mL GS314
HyStem®-HP Hydrogel Trial Kit w/ PEGSSDA, 2.5 mL GS314P
HyStem®-HP Hydrogel Kit, 7.5 mL GS315
HyStem®-HP Hydrogel Kit w/ PEGSSDA, 7.5 mL GS315P
HyStem®-HP Hydrogel Kit, 12.5 mL GS1006

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HyStem®-HP Hydrogel Kit - The growth factor delivery matrix

HyStem-HP hydrogel is fully chemically-defined and is ideal for cell applications whereby the slow, continuous release of growth factors is crucial to re-creating a desired microenvironment. The HyStem-HP Hydrogel Kit contains a combination of thiol-modified hyaluronan and a thiol-modified heparin (Heprasil®), thiol-modified denatured collagen (Gelin-S), and thiolreactive crosslinker, PEGDA (Extralink).

The immobilized heparin in the HyStem-HP hydrogel mimics the heparin sulfate proteoglycans normally present in the extracellular matrix. Heparin forms an ionic bond with proteins and protects them from proteolysis and facilitates their slow release into the cell culture medium. This significantly reduces the amount of growth factor required to trigger cell growth or differentiation compared to when growth factors are added directly to the medium.

  • Growth factors can be mixed into the hydrogels prior to gelation to provide a slow growth factor release depot.
  • Hydrogels are suitable for animal implantation (such as angiogenesis applications and cell or drug delivery), culturing of primary cells, stem cells, and cell lines in the presence of growth factors.
  • Cells can be encapsulated or grown on the hydrogel surface in any format, including culture flasks, 6- to 384-well plates or tissue culture inserts.
  • Hydrogels can be easily customized by the user to possess the desired stiffness and gelation time by manipulating component concentration and mixing ratios.
Reconstituted HyStem-HP components remain liquid at 15 to 37°C. The hydrogel is formed when the crosslinking agent, Extralink® (PEGDA) is added to a mixture of Heprasil® (thiol-modified hyaluronan plus heparin) and Gelin-S® (thiol-modified gelatin). Gelation occurs in about twenty minutes after all three components are mixed. No steps depend on low temperatures or low pH. Diluting the components with phosphate-buffered saline (PBS) or cell-culture medium can increase the gelation time.

3D Cell Recovery Matrix
For application where cell recovery is critical, the alternative crosslinker PEGSSDA is available for use with all HyStem, HyStem-C and HyStem-HP kits. This crosslinker provides the same advantages offered by Extralink with the additional benefit of containing easily reducible internal bonds. This allows for fast, easy recovery of single cells or clusters from the hydrogel for applications like RNA analysis or flow cytometry instead of slow enzymatic methods that can impact cell viability. Researchers are encouraged to contact us to determine the compatibility of particular cell types or culture systems with PEGSSDA.



Storage and Stability Store HyStem-HP and Gelin-S in original vials unopened at -20°C for up to one year.
Store Extralink in the original vial unopened at -20°C for up to one year.
Reconstituted Extralink solutions can be stored at -20°C for ~ one month.
Technical Documents
Technical Data Sheets
GS314 Technical Data Sheet
GS314P Technical Data Sheet
GS315 Technical Data Sheet
GS315P Technical Data Sheet
GS1006 Technical Data Sheet

Product Support
HyStem Hydrogels FAQs
Manuals & Protocols

HyStem® Hydrogels
Components Extralink®
DG Water
References S. Cai, et al. (2005) Injectable glycosaminoglycan hydrogels for controlled release of human basic fibroblast growth factor. Biomaterials, 26, 6054-6067.

D. B. Pike, et al. (2006) Heparin-regulated release of growth factors in vitro and angiogenic response in vivo to implanted hyaluronan hydrogels containing VEGF and bFGF. Biomaterials, 27, 5242–5251.

G. D. Prestwich, et al. (2007) 3-D Culture in Synthetic Extracellular Matrices: New Tissue Models for Drug Toxicology and Cancer Drug Discovery. invited, Adv. Enz. Res., in press (2007).

X. Z. Shu, et al, (2006) Synthesis and Evaluation of Injectable, In Situ Crosslinkable Synthetic Extracellular Matrices (sECMs) for Tissue Engineering. J. Biomed Mater. Res. A, 79A(4), 901-912.


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