Description
Details
Y27632 is a cell-permeable small molecule Rho-associated kinase (ROCK) inhibitor. Y27632 has been found to prevent apoptosis as well as enhance the survival and cloning efficiency of dissociated human ES cells without affecting their self-renewal properties or pluripotency. This molecule has also been shown to enhance survival during the transplantation of ES cell-derived neural precursors and to significantly improve cell recovery after cryopreservation.
Details
Details
| Molecular Weight | 320.26 |
|---|---|
| Molecular Formula | C14H23Cl2N3O |
| Chemical Name | (1R,4r)-4-((R)-1-aminoethyl)-N-(pyridin-4-yl)cyclohexanecarboxamide dihydrochloride |
| CAS Number | 146986-50-7 |
| PubChem Identifier | 9901617 |
| Purity | >98% by HPLC analysis |
| Alternative Names | Y27632 dihydrochloride |
| Solubility | Soluble in DMSO at 50 mM and water at 50 mM |
| Reconstitution | For powder form, reconstitute in DMSO to the desired concentration. For detailed instructions on reconstitution and use in cell culture please reference technical data sheet. |
| Storage and Stability | Solid: Shipped at room temperature. Store at -20°C. Stable for 6 months when stored as directed. 10 mM Solution: Shipped on dry ice. Store at -20°C. Avoid repeated freeze-thaw cycles. Stable for 6 months when stored as directed. |
| References | Koyanagi, M., et al. (2008) Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors. J Neurosci Res 86: 270-280. PMID: 17828770. Watanabe, K., et al. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 25: 681-686. PMID: 17529971. Li, X., et al. (2009) ROCK inhibitor improves survival of cryopreserved serum/feeder-free single human embryonic stem cells. Hum Reprod 24: 580-589. PMID: 19056770. |
| Technical Documents | ST10018 Technical Data Sheet ST10019 Technical Data Sheet ST10020 Technical Data Sheet |
