Description
Details
The TRA-1-60 antibody recognizes a protein expressed on the surface of human embryonal carcinoma (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells. The epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation making it a widely used marker to characterize human ES/iPS cells and to monitor their differentiation.
Sample Data
Figure A: FC analysis on H1 human ES cells at a 1:100 dilution. Red histogram represents TRA-1-60 Antibody and open histogram represents isotype control. A PE-conjugated anti-Mouse IgM was used as the secondary antibody. Figure B: ICC analysis on H1 human ES cells. Cells were stained with TRA-1-60 Antibody at a 1:100 dilution followed by an Alexa 488-conjugated secondary antibody (green). Nuclei were counterstained with DAPI (blue).
Details
Details
| Concentration | 0.5 mg/mL |
|---|---|
| Species Reactivity | Human |
| Host | Mouse Monoclonal |
| Clone | TRA-1-60 |
| Isotype | IgM |
| Immunogen | Human embryonal carcinoma cell line 2102Ep |
| Formulation | Aqueous buffer, 0.09% sodium azide. |
| Storage and Stability | Store at 2-8°C. Stable for 6 months from date of receipt when stored as directed. |
| Applications Tested | Flow Cytometry (FC), Immunocytochemistry/Immunofluorescence (ICC/IF) |
| Recommended Dilutions | Flow Cytometry 1:100 Immunocytochemistry/Immunofluorescence 1:100 It is recommended that the antibody be titrated for optimal performance for each application. |
| Alternative Names | Podocalyxin, MGC138240, PODXL, PCLP, PC, gp200 |
| References | Chin, A.C., et al. (2007) Identification of proteins from feeder conditioned medium that support human embryonic stem cells. J Biotechnol 130: 320-328. PMID: 17544536 Schopperle, W.M., et al. (2007) The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells 25: 723-730. PMID: 17124010 Takahashi, K., et al. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872. PMID: 18035408 |
| Technical Documents | ST11016 Technical Data Sheet |
