Description
Details
PureStem® 7PEND24, NCr-fac expresses markers specific to early adipocytes (PPARG, DLK1, CEBPD), chondrogenic progenitors (PAX9, COL6A1, COL1A1), and neural crest-derived mesenchymal cells (BARX1, LHX8, SNAI2, TWIST1, FOXF1). It exhibits chondrogenic fate upon differentiation in vitro and has a potential adipose fate. This progenitor has been clonally derived from the parental NIH registered Human ES cell line H9 (WA09) (West, M.D. et al, 2008).
Propagation Potential
Progenitor line 7PEND24 is sold at passage 10 (passage 1: original clonal isolate at confluence in 1.9 cm2 well), which corresponds to approximately 13.5 doublings since the first passage and approximately 30 doublings since its original clonal plating. The line displays a finite lifespan in vitro, and when properly maintained will reach at least 10 population doublings.
Propagation Potential
Progenitor line 7PEND24 is sold at passage 10 (passage 1: original clonal isolate at confluence in 1.9 cm2 well), which corresponds to approximately 13.5 doublings since the first passage and approximately 30 doublings since its original clonal plating. The line displays a finite lifespan in vitro, and when properly maintained will reach at least 10 population doublings.
Details
Details
| Quality Control | This embryonic progenitor is negative for HIV (1, 2), HBV, HCV, bacteria, mycoplasma, and fungal contamination. Certificates of analysis are available on request. |
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| Technical Documents | ES-283 Technical Data Sheet |
| References | Sternberg H, et al. (2012) A human embryonic stem cell-derived clonal progenitor cell line with chondrogenic potential and markers of craniofacial mesenchyme. Regen Med. 7(4):481-501. PMID: 22519755
West MD, et al. (2008) The ACTCellerate initiative: large-scale combinatorial cloning of novel human embryonic stem cell derivatives. Regen Med. 3(3):287-308. PMID: 18462054 |