PureStem® ES-1001 GDF11+ Progenitor

PureStem® ES-1001 GDF11+ Progenitor expresses the NKD2, HAND2, TBX3, FOXF1, and the HOX genes: HOXA10 and the relatively distal HOX genes HOXB7 and HOXC8 as well as the hindlimb marker PITX1 (expressed in hindlimb, but not forelimb bud mesenchyme). When these cells are cultured for 21 days in HyStem-C hydrogel with BMP4, the differentiated cells express GDF11, a gene encoding a protein reported to be useful in reversing age-related cardiac hypertrophy in mice (Loffredo et al, 2013). Progenitor ES-1001 was derived from the NIH registered human embryonic stem cell line ESI-017 using a clonal isolation process similar to that previously described (West et al, 2008).

Propagation Potential
PureStem Progenitor ES-1001 is sold at passage 11 (the original clonal isolate confluent in a 1.9 cm2 well was P1), which corresponds to approximately 12 doublings since the first 1.9 cm2 well and approximately 30 doublings since its original clonal plating. The line displays a finite lifespan in vitro, senescing at approximately 70 PD. When used properly this product is guaranteed to scale for a minimum of 10 population doublings.