Description
Details
Mouse recombinant Leukemia Inhibitory Factor (LIF) is a bioactive protein that promotes self-renewal and long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF is also involved in a wide variety of biological processes including neuronal survival and formation, control of stem cell pluripotency, hematopoietic cell proliferation, bone and fat metabolism, and promotion of megakaryocyte production in vivo. Mouse LIF is a 20 kDa protein containing 181 amino acid residues.
Details
Details
| Concentration | 10 μg/mL |
|---|---|
| Source | E. coli |
| Species | Mouse |
| Molecular Mass | 20 kDa |
| Storage and Stability | Store at 2-8°C. Stable for 6 months from date of receipt when stored as directed. |
| Formulation | Sterile liquid in phosphate-buffered saline, 0.02% Tween 20, 1% BSA, and 0.22 μm filtered. |
| Purity | Greater than 98% as determined by SDS-PAGE analysis |
| Endotoxin Level | Less than 1.0 EU/μg of mouse LIF as determined by the LAL assay |
| Biological Activity | Inhibits differentiation of mouse ES cells at 10 ng/mL |
| References | Carpenter, M.K., et al. (2001) Enrichment of neurons and neural precursors from human embryonic stem cells. Exp Neurol 172(2): 383-397. PMID: 11716562 Gearing, D.P., et al. (1987) Molecular cloning and expression of cDNA encoding a murine myeloid leukaemia inhibitory factor (LIF). EMBO J 6(13): 3995-4002. PMID: 3127201 Metcalf, D. (2003) The unsolved enigmas of leukemia inhibitory factor. Stem Cells 21(1): 5-14. PMID: 12529546 |
| Technical Documents |
