PEPTITE-2000® RGD Peptide

PEPTITE-2000® is a synthetic peptide containing the RGD cell attachment sequence found in fibronectin, vitronectin and many other matrix and serum proteins. This RGD peptide has been specifically engineered to spontaneously adhere to a variety of surfaces such as polystyrene (either tissue culture treated or non-treated), hollow fibers made of cellulose, glass, polycarbonate membranes and many other materials. This binding is mediated via a hydroscopic C-terminal sequence. The RGD motif is present at the N-terminal end of the peptide, allowing for optimal cell attachment via integrin receptors. PEPTITE-2000 can be substituted as the primary cell attachment component in a chemically defined animal component-free culture system.

Applications
PEPTITE-2000 is used to coat tissue culture plasticware for enhanced cell attachment and adhesion. The optimal concentration for cell attachment and culture may differ for various cell types. Some experimentation may be required to determine the optimal conditions for individual cell culture systems. A typical working concentration may range from 0.1 to 10 μg/ml. PEPTITE-2000 is not for human use as supplied.