Description
Details
Dorsomorphin inhibits the BMP pathway by targeting the type I receptors ALK2, ALK3, and ALK6. It is also a potent inhibitor of AMPK (Ki = 109 nM) but does not significantly inhibit structurally related kinases such as JAK3, PKCθ, ZAPK, SYK, or PKA. Dorsomorphin has been shown to promote differentiation of neural progenitor cells from human pluripotent stem cells. In addition, it has also been shown to promote differentiation of cardiomyocytes from mouse and human pluripotent stem cells.
Details
Details
| Molecular Weight | 399.49 |
|---|---|
| Molecular Formula | C₂₄H₂₅N₅O |
| Chemical Name | 6-[4-(2-piperidin-1-ylethoxy)phenyl]-3-pyridin-4-ylpyrazolo[1,5-a]pyrimidine |
| CAS Number | 866405-64-3 |
| PubChem Identifier | 11524144 |
| Appearance | Yellow solid |
| Purity | >96% by HPLC analysis |
| Alternative Names | Compound C, AMPK Inhibitor |
| Solubility | Soluble in DMSO at 5 mM |
| Reconstitution | For a 5 mM concentrated stock solution, reconstitute the compound by adding 1.0 mL of DMSO to the entire contents of vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes. |
| Storage and Stability | Solid: Shipped at room temperature. Store at -20°C. Stable for 6 months when stored as directed. Solution: Following reconstitution, store aliquots in tightly sealed vials at -20°C. Avoid repeated freeze-thaw cycles. |
| References | Bain, J., et al. (2007) The selectivity of protein kinase inhibitors: a further update. Biochem J 408: 297-315. PMID: 17850214. Hao, J., et al. (2008) Dorsomorphin, a selective small molecule inhibitor of BMP signaling, promotes cardiomyogenesis in embryonic stem cells. PLoS One 3(8): e2904. PMID: 18682835. Yu, P.B., et al. (2008) Dorsomorphin inhibits BMP signals required for embryogenesis and iron metabolism. Nat Chem Biol 4: 33-41. PMID: 18026094. Zhou, J., et al. (2010) High-efficiency induction of neural conversion in human ESCs and human induced pluripotent stem cells with a single chemical inhibitor of transforming growth factor beta superfamily receptors. Stem Cells 28: 1741-1750. PMID: 20734356. |
| Technical Documents |