Description
Details
The TRA-1-81 Antibody reacts with a pluripotent-stem-cell-specific antigen expressed on the surface of human embryonal carcinoma (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells. The expression of TRA-1-81 antigen is stage-specific; as human EC and ES/iPS cells undergo differentiation, expression of TRA-1-81 antigen is lost, making it a widely used marker to characterize human ES/iPS cells and to monitor their differentiation.
Sample Data
Figure A: FC analysis on H1 human ES cells at a 1:100 dilution.Red histogram represents TRA-1-81 Antibody and open histogram represents isotype control. A PE-conjugated anti-Mouse IgM was used as the secondary antibody. Figure B: ICC analysis on H1 human ES cells. Cells were stained with TRA-1-81 Antibody at a 1:100 dilution followed by an Alexa 488-conjugated secondary antibody (green). Nuclei were counterstained with DAPI (blue).
Details
Details
| Concentration | 0.5 mg/mL |
|---|---|
| Species Reactivity | Human |
| Host | Mouse Monoclonal |
| Clone | TRA-1-81 |
| Isotype | IgM |
| Immunogen | Human embryonal carcinoma cell line 2102Ep |
| Formulation | Aqueous buffer, 0.09% sodium azide. |
| Storage and Stability | Store at 2-8°C. Stable for 6 months from date of receipt when stored as directed. |
| Applications Tested | Flow Cytometry (FC), Immunocytochemistry/Immunofluorescence (ICC/IF) |
| Recommended Dilutions | Flow Cytometry 1:100 Immunocytochemistry/Immunofluorescence 1:100 It is recommended that the antibody be titrated for optimal performance for each application. |
| Alternative Names | Podocalyxin, MGC138240, PODXL, PCLP, PC, gp200 |
| References | Andrews, P.W., et al. (1984) Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma 3: 347-361. PMID: 6396197 Henderson, J.K., et al. (2002) Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 20: 329-337. PMID: 12110702 Schopperle, W.M., et al. (2007) The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells 25: 723-730. PMID: 17124010 |
| Technical Documents | ST11017 Technical Data Sheet |
