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SSEA-1 anti-Human/Mouse Antibody, 100 µL

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Stage-specific embryonic antigen-1 (SSEA-1) is expressed upon the surface of early mouse embryos, murine embryonal carcinoma, murine embryonic stem, and murine & human germ (EG) cells.
SKU: ST11013

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Stage-specific embryonic antigen-1 (SSEA-1) is a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma (EC), murine embryonic stem (ES), and murine and human germ (EG) cells. No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. SSEA-1 is associated with cell adhesion, migration and differentiation.

Sample Data

antibody icc analysis

Figure A: FC analysis of SSEA-1 Antibody on mouse ES cells at a 1:100 dilution.
Green histogram represents SSEA-1 Antibody and open histogram represents isotype control. A PE-conjugated anti-Mouse IgM was used as the secondary antibody. Figure B: ICC analysis on mouse ES cells. Cells were stained with SSEA-1 Antibody at a 1:100 dilution followed by an Alexa Fluor 594-conjugated secondary antibody (red). Nuclei were counterstained with DAPI (blue).



Concentration 0.5 mg/mL
Species Reactivity Human, Mouse
Host Mouse Monoclonal
Clone MC-480
Isotype IgM
Immunogen F9 tetracarcinoma stem cells (X-irradiated)
Formulation Aqueous buffer, 0.09% sodium azide.
Storage and Stability Store at 2-8°C. Stable for 6 months from date of receipt when stored as directed.
Applications Tested Flow Cytometry (FC), Immunocytochemistry/Immunofluorescence (ICC/IF)
Recommended Dilutions Flow Cytometry 1:100
Immunocytochemistry/Immunofluorescence 1:100
It is recommended that the antibody be titrated for optimal performance for each application.
Alternative Names CD15, Stage-specific embryonic antigen 1
References Brambrink, T., et al. (2008) Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2: 151-159. PMID: 18371436

Draper, J.S., et al. (2002) Surface antigens of human embryonic stem cells: changes upon differentiation in culture. J Anat 200: 249-258. PMID: 12033729
Technical Documents ST11013 Technical Data Sheet


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