Description
Details
The Sox2 or SRY (sex determining region Y)-box 2 transcription factor contains a high motility group DNA-binding domain which functions to bind and open the DNA helix, facilitating the actions of other transcription factors. The protein is essential for maintaining the self-renewal and pluripotency capabilities of embryonic stem (ES) cells and therefore is commonly used to characterize stem cell populations. Sox2 is an important functional marker of neural precursor cells and plays a critical role in neuronal differentiation.
Details
Details
| Concentration | 0.5 mg/mL |
|---|---|
| Species Reactivity | Human, Mouse |
| Host | Rat Monoclonal |
| Isotype | IgG2a, κ |
| Immunogen | Human Sox2 Recombinant Protein |
| Formulation | Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2 |
| Storage and Stability | Aliquot and store at -20°C. Avoid repeated freeze-thaw cycles. Stable for 6 months from date of receipt when stored as directed. |
| Applications Tested | Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (FC) |
| Recommended Dilutions | Flow Cytometry 1:100 Immunocytochemistry/Immunofluorescence 1:100 It is recommended that the antibody be titrated for optimal performance for each application. |
| Alternative Names | SRY (sex determining region Y)-box 2 |
| References | Cavallaro, M., et al. (2008) Impaired generation of mature neurons by neural stem cells from hypomorphic Sox2 mutants. Development 135: 541-557. PMID: 18171687. Fong, H., et al. (2008) Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells. Stem Cells 26: 1931-1938. PMID: 18388306. Graham, V., et al. (2003) SOX2 functions to maintain neural progenitor identity. Neuron 39: 749-765. PMID: 12948443. Takahashi, K., et al. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872. PMID: 18035408. |
| Technical Documents |
