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Nestin anti-Human Antibody, 100 μL

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Nestin is a class VI intermediate filament protein expressed in stem cells.
SKU: ST11023

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Nestin is a class VI intermediate filament protein expressed in stem cells of the developing central nervous system (CNS) but not in mature CNS cells. Its expression is widely used as a predominant marker for neural lineage cells derived from human ES and iPS cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.



Concentration 0.5 mg/mL
Species Reactivity Human
Host Mouse Monoclonal
Clone 10C2
Isotype IgG1
Immunogen A recombinant 150 amino acid fragment from human Nestin conjugated to glutathione S transferase.
Formulation Aqueous buffer, 0.09% sodium azide, (pH 7.2).
Storage and Stability Store at 2-8°C. Stable for 6 months from date of receipt when stored as directed.
Applications Tested Immunocytochemistry/Immunofluorescence (ICC/IF)
Recommended Dilutions Immunocytochemistry/Immunofluorescence 1:100
Western Blot 1:100
It is recommended that the antibody be titrated for optimal performance for each application.
References Lendahl, U., et al. (1990) CNS stem cells express a new class of intermediate filament protein. Cell 60(4): 585-595. PMID: 1689217.

Carpenter, M.K., et al. (2001) Enrichment of neurons and neural precursors from human embryonic stem cells. Exp Neurol 172(2): 383-397. PMID: 11716562.

Gilyarov, A.V. (2008) Nestin in central nervous system cells. Neurosci Behav Physiol 38(2): 165-169. PMID: 18197384.
Technical Documents

ST11023 Technical Data Sheet


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