Description
Details
Nestin is a class VI intermediate filament protein expressed in stem cells of the developing central nervous system (CNS) but not in mature CNS cells. Its expression is widely used as a predominant marker for neural lineage cells derived from human ES and iPS cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.
Details
Details
| Concentration | 0.5 mg/mL |
|---|---|
| Species Reactivity | Human |
| Host | Mouse Monoclonal |
| Clone | 10C2 |
| Isotype | IgG1 |
| Immunogen | A recombinant 150 amino acid fragment from human Nestin conjugated to glutathione S transferase. |
| Formulation | Aqueous buffer, 0.09% sodium azide, (pH 7.2). |
| Storage and Stability | Store at 2-8°C. Stable for 6 months from date of receipt when stored as directed. |
| Applications Tested | Immunocytochemistry/Immunofluorescence (ICC/IF) |
| Recommended Dilutions | Immunocytochemistry/Immunofluorescence 1:100 Western Blot 1:100 It is recommended that the antibody be titrated for optimal performance for each application. |
| References | Lendahl, U., et al. (1990) CNS stem cells express a new class of intermediate filament protein. Cell 60(4): 585-595. PMID: 1689217. Carpenter, M.K., et al. (2001) Enrichment of neurons and neural precursors from human embryonic stem cells. Exp Neurol 172(2): 383-397. PMID: 11716562. Gilyarov, A.V. (2008) Nestin in central nervous system cells. Neurosci Behav Physiol 38(2): 165-169. PMID: 18197384. |
| Technical Documents |
