Description
Details
PureStem® SM30, NCr-fac Progenitor expresses axial and appendicular level-derived mesenchymal cell markers including FGF18 and ITGB4 and no HOX gene expression. This PureStem Progenitor is intensely chondrogenic when differentiated in vitro using PureStem’s chodrogenic differentiation kits, expressing high levels of cartilage markers including COL2A1, LECT1, MATN4, EPYC COL9A1 and chondroadherin (CHAD). It also expresses osteogenic fate when differentiated using the PureStem osteogenic differentiation kit. PureStem SM30, NCr-fac Progenitor has been clonally derived from the parental NIH registered Human ES cell line H9 (WA09) (West, M.D. et al, 2008).
Propagation Potential
PureStem Mesenchymal Progenitor SM30 is sold at passage 11 (passage 1: original clonal isolate at confluence in 1.9 cm2 well), which corresponds to approximately 13.5 doublings since the first passage and approximately 30 doublings since its original clonal plating. The line displays a finite lifespan in vitro, and when properly maintained may reach 70 population doublings.
Propagation Potential
PureStem Mesenchymal Progenitor SM30 is sold at passage 11 (passage 1: original clonal isolate at confluence in 1.9 cm2 well), which corresponds to approximately 13.5 doublings since the first passage and approximately 30 doublings since its original clonal plating. The line displays a finite lifespan in vitro, and when properly maintained may reach 70 population doublings.
Details
Details
| Applications | Chondrogenesis and osteogenesis research and cartilage and bone-related diseases research. |
|---|---|
| Quality Control | This line is negative for HIV (1, 2), HBV, HCV, bacteria, mycoplasma, and fungal contamination. Certificates of analysis are available on request. |
| Technical Documents | ES-256 Technical Data Sheet |
| References | West MD, et al. (2008) The ACTCellerate initiative: large-scale combinatorial cloning of novel human embryonic stem cell derivatives. Regen Med. 3(3):287-308. PMID: 18462054 |