Description
Details
PureStem® SK11, NCr-fac Progenitor expresses chondrogenic (COL1A1, VCAN, SOX9, COL6A1) and osteogenic (RUNX2, ALPL, SPP1) markers. In addition, it expresses markers of axial and appendicular level-derived mesenchymal cells (SIX1, TWIST1,SNAI2). This embryonic progenitor exhibits chondrogenic and osteogenic fates upon differentiation in vitro when using PureStem Differentiation Kits. This Progenitor was clonally derived from the parental NIH registered Human ES cell line H9 (WA09) (West, M.D. et al, 2008).
Propagation Potential
PureStem Mesenchymal Progenitor SK11 is sold at passage 11 (passage 1: original clonal isolate at confluence in 1.9 cm2 well), which corresponds to approximately 13.5 doublings since the first passage and approximately 30 doublings since its original clonal plating. The line displays a finite lifespan in vitro, and when properly maintained may reach 70 population doublings.
Propagation Potential
PureStem Mesenchymal Progenitor SK11 is sold at passage 11 (passage 1: original clonal isolate at confluence in 1.9 cm2 well), which corresponds to approximately 13.5 doublings since the first passage and approximately 30 doublings since its original clonal plating. The line displays a finite lifespan in vitro, and when properly maintained may reach 70 population doublings.
Details
Details
| Applications | Chondrogenesis and osteogenesis research and cartilage and bone-related diseases research. |
|---|---|
| Quality Control | This line is negative for HIV (1, 2), HBV, HCV, bacteria, mycoplasma, and fungal contamination. Certificates of analysis are available on request. |
| Technical Documents | ES-250 Technical Data Sheet |
| References | West MD, et al. (2008) The ACTCellerate initiative: large-scale combinatorial cloning of novel human embryonic stem cell derivatives. Regen Med. 3(3):287-308. PMID: 18462054 |