Description
Details
PureStem® ES-209, Progenitor expresses markers of intermediate mesoderm particularly resembling early renal progenitors, metanephric mesenchyme and S-shaped body markers (PAX2, PAX8, LHX1, HNF1B and
JAG1). It also highly expresses the mesodermal marker GALR2 and markers of mural cells, smooth muscle cells (ACTA2, AEBP1, TAGN, CNN1) and Pericytes (KCNJ8, ACTA2, PDGFB, HBEGF). This progenitor is clonally derived from the parental NIH registered Human ES cell line H9 (WA09) (West, M.D. et al, 2008).
Propagation Potential
PureStem Epithelial Progenitor E164 is sold at passage 11 (passage 1: original clonal isolate at confluence in 1.9 cm2 well), which corresponds to approximately 16.5 doublings since the first passage and approximately 30 doublings since its original clonal plating. The line displays a finite lifespan in vitro, and when properly maintained may reach 70 population doublings.
Propagation Potential
PureStem Epithelial Progenitor E164 is sold at passage 11 (passage 1: original clonal isolate at confluence in 1.9 cm2 well), which corresponds to approximately 16.5 doublings since the first passage and approximately 30 doublings since its original clonal plating. The line displays a finite lifespan in vitro, and when properly maintained may reach 70 population doublings.
Details
Details
| Applications | Epithelial research and renal disease research. |
|---|---|
| Quality Control | This line is negative for HIV (1, 2), HBV, HCV, bacteria, mycoplasma, and fungal contamination. Certificates of analysis are available on request. |
| Technical Documents | ES-209 Technical Data Sheet |
| References | West MD, et al. (2008) The ACTCellerate initiative: large-scale combinatorial cloning of novel human embryonic stem cell derivatives. Regen Med. 3(3):287-308. PMID: 18462054 |