Description
Details
PureStem® E15, Meso-prx/latp Progenitor expresses chondrogenic markers (SOX9, VCAN, COL6A2, COL1A1) and markers of axial and appendicular derived mesenchymal cells (TFAP2A, CDH2, SIX1). PureStem Progenitor E68 was derived from the parental hES cell line MA03 as described (West, M.D. et al, 2008).
Propagation Potential
PureStem Mesenchymal Progenitor E15 is sold at passage 13 (passage 1: original clonal isolate at confluence in 1.9 cm2 well), which corresponds to approximately 16.5 doublings since the first passage and approximately 33 doublings since its original clonal plating. The line displays a finite lifespan in vitro, and when properly maintained may reach 80 population doublings.
Propagation Potential
PureStem Mesenchymal Progenitor E15 is sold at passage 13 (passage 1: original clonal isolate at confluence in 1.9 cm2 well), which corresponds to approximately 16.5 doublings since the first passage and approximately 33 doublings since its original clonal plating. The line displays a finite lifespan in vitro, and when properly maintained may reach 80 population doublings.
Details
Details
| Applications | Chondrogenesis research and cartilage-related diseases research. |
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| Quality Control | This line is negative for HIV (1, 2), HBV, HCV, bacteria, mycoplasma, and fungal contamination. Certificates of analysis are available on request. |
| Technical Documents | ES-98 Technical Data Sheet |
| References | West MD, et al. (2008) The ACTCellerate initiative: large-scale combinatorial cloning of novel human embryonic stem cell derivatives. Regen Med. 3(3):287-308. PMID: 18462054 |