Description
Details
BioLite™ surface markers allow for direct immunofluorescent staining of live, unfixed pluripotent stem cell populations. The antibodies undergo meticulous application testing and validation to ensure they are sterile, non-toxic, contain no sodium azide, and are free of culture pathogens. Colonies stained with BioLite antibodies are able to continue in culture with no adverse effect on proliferation and differentiation potential when compared to untreated cells.
The TRA-1-81 antibody reacts with a pluripotent-stem-cell-specific antigen expressed on the surface of human embryonal carcinoma (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells. The expression of TRA-1-81 antigen is stage-specific; as human EC and ES cells undergo differentiation, expression of TRA-1-81 antigen is lost, making it a widely used marker to characterize human ES cells and to monitor their differentiation.
Details
Details
| Concentration | 0.5 mg/mL |
|---|---|
| Species Reactivity | Human |
| Host | Mouse Monoclonal |
| Clone | TRA-1-81 |
| Isotype | IgM, κ |
| Immunogen | Human embryonal carcinoma cell line 2102Ep |
| Formulation | 0.2 μm filter sterilized phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/μg of the protein (<0.01 ng/μg of the protein) as determined by the LAL test. Formulation is free from bacteria, fungi, and mycoplasma. |
| Storage and Stability | Store at 2-8°C protected from light. Stable for 6 months from date of receipt when stored as directed. The BioLite format contains no preservative and therefore must be handled under aseptic conditions. |
| Applications Tested | Immunofluorescence (IF) on live, unfixed human ES cells |
| Recommended Dilutions | Immunofluorescence 1:100 Flow Cytometry 1:100 It is recommended that the antibody be titrated for optimal performance for each application. |
| Alternative Names | Podocalyxin, MGC138240, PODXL, PCLP, PC, gp200 |
| References | Andrews, P.W., et al. (1984) Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma 3: 347-361. PMID: 6396197 Henderson, J.K., et al. (2002) Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 20: 329-337. PMID: 12110702 |
| Technical Documents | ST11008 Technical Data Sheet |
