Description
Details
PureStem® MEL2, NCr-fac Progenitor expresses chondrogenic (NKX3-2, SOX9, COL1A1) and osteogenic (SATB2, ALPL, MSX2) markers. In addition, it expresses markers of neural crest and lateral plate-derived head and limb mesenchymal cells (FRZB, HAND2, DLX5, DLX6, TWIST1, FOXD1, MSX2, TFAP2A, SOX9). MEL2 demonstrates robust chondrogenic differentiation in vitro when using PureStem differentiation kits and reagents. This progenitor has been clonally derived from the parental NIH registered Human ES cell line H9 (WA09) (West, M.D. et al, 2008).
Propagation Potential
PureStem Mesenchymal Progenitor MEL2 is sold at passage 17 (passage 1: original clonal isolate at confluence in 1.9 cm2 well), which corresponds to approximately 13.5 doublings since the first passage and approximately 39 doublings since its original clonal plating. The line displays a finite lifespan in vitro, and when properly maintained may exceed 55 population doublings.
Propagation Potential
PureStem Mesenchymal Progenitor MEL2 is sold at passage 17 (passage 1: original clonal isolate at confluence in 1.9 cm2 well), which corresponds to approximately 13.5 doublings since the first passage and approximately 39 doublings since its original clonal plating. The line displays a finite lifespan in vitro, and when properly maintained may exceed 55 population doublings.
Details
Details
| Applications | Chondrogenesis and osteogenesis research and cartilage and bone-related diseases research. |
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| Quality Control | This line is negative for HIV (1, 2), HBV, HCV, bacteria, mycoplasma, and fungal contamination. Certificates of analysis are available on request. |
| References | West MD, et al. (2008) The ACTCellerate initiative: large-scale combinatorial cloning of novel human embryonic stem cell derivatives. Regen Med. 3(3):287-308. PMID: 18462054 |