Description
Details
A more clinically-relavant co-culture assay
The VascuNet™ Pericyte Co-Culture Assay is a novel angiogenesis model that includes HUVECs and pericytes (PC-Ms) to study the effects of drug and therapeutic compounds on vascularization in a 96-well plate. PC-M cells are derived from a proprietary differentiation method using the ESI-017 Human Embryonic Stem Cell Line. Cells and media components undergo strict quality screening to ensure reproducibility. Networks are maintained for up to four to six days, allowing for a more extensive and clinically-relevant model system for studying the effects of compounds on tube formation.Features
- VascuNet tube networks approximate in vivo vessels more closely than other systems
- Allows for long-term in vitro analyses of pro- and anti-angiogenic compounds on established vessels
- Pericytes are hESC-derived ensuring easy expansion and reproducible results
Details
Details
Format | 96-well plate |
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Sample Types | Small molecules, recombinant proteins, DNA/RNA constructs, or conditioned media |
Assay Time | Tube networks are maintained for at least 4 days. |
Storage and Stability | All kit components are stable for a minimum of 3 months from date of receipt when stored as directed. |
Components | For a complete list of kit components, please download the EM-2202 Technical Data Sheet |
Applications | Screening and the assessment of pro- or anti-angiogenic compounds |
Technical Documents | Product Support VascuNet™ Pericyte Co-Culture Assay Protocol |
References | Gerhardt, H. and Betsholtz, C. (2003) Endothelial-pericyte interactions in angiogenesis. Cell Tissue Res 314: 15–23. Hamilton, N.B., et al. (2010) Pericyte-mediated regulation of capillary diameter: a component of neurovascular coupling in health and disease. Front Neuroenergetics 2: 5. Stratman, A.N., et al. (2009) Pericyte recruitment during vasculogenic tube assembly stimulates endothelial basement membrane matrix formation. Blood 114: 5091–5101. von Tell, D., et al. (2006) Pericytes and vascular stability. Exp Cell Res 312: 623–629. |