Description
Details
BIX01294 is a very specific inhibitor of the G9a (IC50 = 1.9 μM) and G9a-like (IC50 = 0.7 μM) histone methyltransferases which modulates H3K9 levels. It has been shown to enhance reprogramming of mouse embryonic fibroblasts or fetal neural progenitor cells to induced pluripotent stem cells without using c-Myc and Sox2.
Details
Details
Molecular Weight | 600.02 |
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Molecular Formula | C28H38N6O2.3HCl |
Chemical Name | 2-(Hexahydro-4-methyl-1H-1,4-diazepin-1-yl)-6,7-dimethoxy-N-[1-(phenylmethyl)-4-piperidinyl]-4-quinazolinamine trihydrochloride |
CAS Number | 1392399-03-9 |
PubChem Identifier | 46945860 |
Appearance | Yellow solid |
Purity | >99% by HPLC analysis |
Alternative Names | BIX01294 Trihydrochloride Hydrate |
Solubility | Soluble in DMSO at 100 mM and water at 100 mM |
Reconstitution | For a 10 mM concentrated stock solution, reconstitute the compound by adding 333 μL of DMSO to the entire contents of vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes. |
Storage and Stability | Solid: Shipped at room temperature. Store at -20°C. Stable for 6 months when stored as directed. Solution: Following reconstitution, store aliquots in tightly sealed vials at -20°C. Avoid repeated freeze-thaw cycles. |
References | Kubicek, S., et al. (2007) Reversal of H3K9me2 by a small-molecule inhibitor for the G9a histone methyltransferase. Mol Cell 25: 473-481. PMID: 17289593. Shi, Y., et al. (2008) Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds. Cell Stem Cell 3(5): 568-574. PMID: 18983970. Shi, Y., et al. (2008) A combined chemical and genetic approach for the generation of induced pluripotent stem cells. Cell Stem Cell 2: 525-528. PMID: 18522845 |
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