Description
Details
ESI BIO offers the highest quality human embryonic stem cell
(hESC) lines that are among the best-characterized and documented
lines commercially available today. The ESI cell lines
are NIH registered and backed with donor history and testing
information in best compliance with current Good Tissue
Practice (cGTP) and conform to Global Ethical Standards and
Clinical Cell Regulations. ESI hESCs were derived under current
Good Manufacturing Practice (cGMP) conditions on human
fibroblast feeder layers and are available in both research and
clinical-grade formats. For most investigators, research banks of
the ESI hESC lines provide a convenient entry point, while GMP
compliant banks of these same cells create seamless translation
into clinically relevant studies.
The ESI hESCs are well characterized in regards to genomic integrity, pluripotency status, and culturing behavior. Each line has been karyotyped, STR fingerprinted, has high viability thawing recovery, is characterized by pluripotency marker expression, and is free of mycoplasma, pathogens, bacteria, yeast, and fungus. In addition to the standard pluripotency and karyotype analysis, the complete genome sequence is available for purchase.
The ESI hESCs are well characterized in regards to genomic integrity, pluripotency status, and culturing behavior. Each line has been karyotyped, STR fingerprinted, has high viability thawing recovery, is characterized by pluripotency marker expression, and is free of mycoplasma, pathogens, bacteria, yeast, and fungus. In addition to the standard pluripotency and karyotype analysis, the complete genome sequence is available for purchase.

Details
Details
Format | Research grade |
---|---|
Culture Conditions | Feeder independent in defined medium |
Passage Number | < 25 |
Storage | Liquid nitrogen |
Expanded and Banked by | BioTime, Inc. |
Technical Documents | ESI-017 Technical Data Sheet ESI-035 Technical Data Sheet ESI-049 Technical Data Sheet ESI-051 Technical Data Sheet ESI-053 Technical Data Sheet
Product Support EDTA-based Passaging of ESI Human Embryonic Stem Cells Protocol Brochures |
References | Crook, J.M., et al. (2007) The generation of six clinical-grade human embryonic stem cell lines. Cell Stem Cell 1: 490-494. PMID: 18938745. Funk, W.D., et al. (2012) Evaluating the genomic and sequence integrity of human ES cell lines; comparison to normal genomes. Stem Cell Res 8(2): 154-164. PMID: 22265736. |