Description
Details
Thiazovivin is a selective inhibitor of Rho-associated coiled-coil containing protein kinase (ROCK), a serine/threonine kinase that plays a role in cell polarity, contraction, and actin cytoskeleton reorganization. Thiazovivin has been shown to promote survival of human ES cells during dissociation by stabilizing E-cadherin and to improve cell attachment. It has also been shown to greatly enhance the efficiency of fibroblast reprogramming to generate iPS cells when used in combination with SB431542 and PD0325901.
Details
Details
| Molecular Weight | 311.36 |
|---|---|
| Molecular Formula | C15H13N5OS |
| Chemical Name | N-benzyl-2-(pyrimidin-4-ylamino)-1,3-thiazole-4-carboxamide |
| CAS Number | 1226056-71-8 |
| PubChem Identifier | 46209426 |
| Appearance | Off-white solid |
| Purity | >97% by HPLC analysis |
| Solubility | Soluble in DMSO at 100 mM |
| Reconstitution | For a 10 mM concentrated stock solution, reconstitute the compound by adding 642 µL of DMSO to the entire contents of vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes. |
| Storage and Stability | Solid: Shipped at room temperature. Store at -20°C. Stable for 6 months when stored as directed. Solution: Following reconstitution, store aliquots in tightly sealed vials at -20°C. Avoid repeated freeze-thaw cycles. |
| References | Xu, Y., et al. (2010) Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc Natl Acad Sci USA 107: 8129-8134. PMID: 20406903. Lin, T., et al. (2009) A chemical platform for improved induction of human iPSCs. Nat Methods 6: 805-808. PMID: 19838168. |
| Technical Documents |