Description
Details
SP600125 is a selective inhibitor of c-Jun N-terminal kinase (JNK). SP600125 competitively and reversibly inhibits JNK1, 2 and 3 (IC50 = 40 - 90 nM) with negligible activity at ERK2 and p38β. Shown to have reduced selectivity over other protein kinases under certain conditions. Protects renal tubular epithelial cells against ischemia and reperfusion-induced apoptosis.
Details
Details
| Molecular Weight | 220.23 |
|---|---|
| Molecular Formula | C14H8N2O |
| Chemical Name | anthra(1,9-cd)pyrazol-6(2H)-one |
| CAS Number | 129-56-6 |
| PubChem Identifier | 8515 |
| Appearance | Yellow solid |
| Purity | >99% by HPLC analysis |
| Alternative Names | 1,9-Pyrazoloanthrone |
| Solubility | Soluble in DMSO at 100 mM |
| Reconstitution | For a 10 mM concentrated stock solution, reconstitute the compound by adding 2.27 mL of DMSO to the entire contents of vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes. |
| Storage and Stability | Solid: Shipped at room temperature. Store at -20°C. Stable for 6 months when stored as directed. Solution: Following reconstitution, store aliquots in tightly sealed vials at -20°C. Avoid repeated freeze-thaw cycles. |
| References | Bennett, B.L., et al. (2001) SP600125, an anthrapyrazolone inhibitor of Jun N-terminal kinase. Proc Natl Acad Sci USA 98: 13681-13686. PMID: 11717429. Zhao, Y.F., et al. (2013) Activation of JNKs is essential for BMP9-induced osteogenic differentiation of mesenchymal stem cells. BMB Rep 46: 422-427. PMID: 23977991. |
| Technical Documents | ST10025 Technical Data Sheet |
