Description
Details
Forskolin is a cell permeable activator of adenylyl cyclase (IC50 = 41 nM), resulting in higher levels of cyclic AMP (cAMP) in the cell. It has been used to potentiate neuron differentiation as well as maintain human ES cells in a naïve or ground state. Forskolin has been shown to enable chemical reprogramming of mouse embryonic fibroblasts to iPS cells in combination with CHIR99021, Tranylcypromine, Valproic Acid, 3-Deazaneplanocin A, and RepSox.
Details
Details
| Molecular Weight | 410.5 |
|---|---|
| Molecular Formula | C22H34O7 |
| Chemical Name | [3R-(3α,4aβ,5β,6β,6aα,10α,10aβ,10bα)]-5-(Acetyloxy)-3-ethenyldodecahydro-6,10,10b-trihydroxy-3,4a,7,7,10a-pentamethyl-1H-naphtho[2,1-b]pyran-1-on |
| CAS Number | 1392399-03-9 |
| PubChem Identifier | 47936 |
| Appearance | White to off-white solid |
| Purity | >99% by HPLC analysis |
| Alternative Names | Colforsin, Coleonol, Boforsin |
| Solubility | Soluble in DMSO at 25 mM |
| Reconstitution | For a 10 mM concentrated stock solution, reconstitute the compound by adding 2.4 mL of DMSO to the entire contents of vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes. |
| Storage and Stability | Solid: Shipped at room temperature. Store at -20°C. Stable for 6 months when stored as directed. Solution: Following reconstitution, store aliquots in tightly sealed vials at -20°C. Avoid repeated freeze-thaw cycles. |
| References | Hanna, J., et al. (2010) Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs. Proc Natl Acad Sci USA 107: 9222-9227. PMID: 20442331. Hou, P., et al. (2013) Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds. Science 341: 651-654. PMID: 23868920. Kim, S.S., et al. (2005) cAMP induces neuronal differentiation of mesenchymal stem cells via activation of extracellular signal-regulated kinase/MAPK. Neuroreport 16: 1357-1361. PMID: 16056139. Robbins, J.D., et al. (1996) Forskolin carbamates: binding and activation studies with type I adenylyl cyclase. J Med Chem 39: 2745-2752. PMID: 8709105. |
| Technical Documents |