Description
Details
Selective inhibitor of TGF-β type I receptor ALK5 kinase, type I activin/nodal receptor ALK4 and type I nodal receptor ALK7 (IC50 values are 12, 45 and 7.5 nM respectively). A83-01 blocks phosphorylation of Smad2 and inhibits TGF-β-induced epithelial-to-mesenchymal transition. Only weakly inhibits ALK-1, -2, -3, -6 and MAPK activity. A83-01 has been shown to inhibit differentiation of rat iPSCs and increase clonal expansion efficiency. It helps maintain homogeneity and long-term in vitro self-renewal of human iPSCs.
Details
Details
| Molecular Weight | 421.52 |
|---|---|
| Molecular Formula | C25H19N5S |
| Chemical Name | 3-(6-Methyl-2-pyridinyl)-N-phenyl-4-(4-quinolinyl)-1H-pyrazole-1-carbothioamide |
| CAS Number | 909910-43-6 |
| PubChem Identifier | 16218924 |
| Appearance | Pale yellow solid |
| Purity | >96% by HPLC Analysis |
| Solubility | Soluble in DMSO at 50 mM |
| Reconstitution | For powder form, reconstitute in DMSO to the desired concentration. For detailed instructions on reconstitution and use in cell culture please reference technical data sheet. |
| Storage and Stability | Solid: Shipped at room temperature. Store at -20°C. Stable for 6 months when stored as directed. Solution: Following reconstitution, store aliquots in tightly sealed vials at -20°C. Avoid repeated freeze-thaw cycles. |
| References | Hoberg, M., et al. (2008) Attachment to laminin-111 facilitates transforming growth factor β-induced expression of matrix metalloproteinase-2 in synovial fibroblasts. Ann Rheum Dis 66: 446-451. PMID: 17124250. Li, W., et al. (2009) Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors. Cell Stem Cell 4: 16-19. PMID: 19097958. Tojo, M., et al. (2005) The ALK-5 inhibitor A-83-01 inhibits Smad signaling and epithelial-to-mesenchymal transition by transforming growth factor-beta. Cancer Sci 96: 791-800. PMID: 16271073. |
| Technical Documents |
